Physiology & Pharmacology
Ezzat Nourizadeh
Abstract
Background: Cancer is the second cause of death in the world. Worldwide, many cancers cause varying degrees of morbidity. Also, side effects of chemical drugs used to treat various cancers have been reported. Considering this importance, the purpose of this research is to investigate the anticancer effects ...
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Background: Cancer is the second cause of death in the world. Worldwide, many cancers cause varying degrees of morbidity. Also, side effects of chemical drugs used to treat various cancers have been reported. Considering this importance, the purpose of this research is to investigate the anticancer effects of different Scutellaria species on different human cancer cells.Materials and Methods: In this descriptive study, the method of data collection was computerized and from valid internet databases, and sources such as Google Scholar/Pubmed and other tools were used. Clinical data on the diagnosis, treatment and prevention of cancer by herbs. The mint family Scutellaria is summarized and data are extracted from various research reports and other authoritative sources.Results: Among the active chemical compounds in the genus Scutellaria are flavonoids, which are considered the most important of these compounds.The flavonoids isolated from this plant prevent the development of cancer with their antioxidant, anti-mutagenic activities and by stopping the cell cycle.Conclusion: Although the anticancer properties of Scutellaria have been shown, deciding whether Scutellaria can be used as an anticancer agent in the clinic depends on many factors and needs to be investigated.
Biotechnology & nanotechnology
Ezzat Nourizadeh
Volume 28, Issue 5 , November and December 2021, , Pages 775-789
Abstract
Introduction: Leishmania (L.) infantum is the etiologic cause of visceral leishmaniasis in Iran. Efficient vaccines and diagnosis methods are required to control leishmaniasis. The aim of this study is produce and optimize monoclonal antibodies against promastigotes forms of L. infantum antigen.
Materials ...
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Introduction: Leishmania (L.) infantum is the etiologic cause of visceral leishmaniasis in Iran. Efficient vaccines and diagnosis methods are required to control leishmaniasis. The aim of this study is produce and optimize monoclonal antibodies against promastigotes forms of L. infantum antigen.
Materials and Methods: The mice were vaccinated with the L. infantum antigen and their antibody titers were determined by the ELISA method. Spleen cells of the most immune mouse were fused with SP2/0 in the presence of Poly Ethylene Glycol.The effect of supernatant of SP2/0 and mice peritoneum macrophage cells culture (SSMCC) on hybridoma cell proliferation was studied.
Results: Among the 12 fusion, a total of 26 monoclonal were positive.12 of which had acceptable optical absorbance in OD 450 nm. Finally, 4 clones, designated as 8D2 FVI6, 8D2 FVI3, 6G2 FV4 and 6G2 FV3. From these hybrids, anti-promastigotes L. infantum monoclonal antibodies were obtained. SSMCC was shown to play a key role in hybridoma proliferation and of mAb production. It seemed that SSMCC is rich of growth factors.
Conclusion: It seems in the near future, this SCCSM can be used as a growth factor for cancerous and non-cancerous cells in research centers at a wider level.
Microbiology
sima nobari; Ezzat Nourizadeh
Volume 28, Issue 3 , May and June 2021, , Pages 449-456
Abstract
Introduction: Leishmaniasis is a disease in tropical regions, that includes a wide range of clinical protests, from skin lesions to fatal visceral infections. A2 gene is accounted as one of the most reliable genetic factors cause visceral form. This gene is a single copy without functional expression ...
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Introduction: Leishmaniasis is a disease in tropical regions, that includes a wide range of clinical protests, from skin lesions to fatal visceral infections. A2 gene is accounted as one of the most reliable genetic factors cause visceral form. This gene is a single copy without functional expression in the species causing skin form of the disease such as L. major and L. tropica. The aim of our study is to evaluate A2 gene sequence in the strains that cause skin form of disease and compare it with other exception strains of the Leishmania tropica strains in Iran.
Materials and Methods: Leishmania species were detected using ITS1 and ITS2. The parasites were injected into BALB/c mice and monitored of footpad inflammation in BALB/c mice. after a certain period of time the mice were killed and their visceral organs were examined for the presence of parasites. Finally, A2 gene sequence analyzed.
Results: A2 gene in a strain causing visceral form was different to a gel electrophoresis pattern of skin form causing strains. Also, the gel electrophoresis pattern of A2 gene in strains causing skin form was different to previous reports of cutaneous leishmaniasis.
Conclusion: The results of this recent experiment showed that the gel electrophoresis pattern of A2 gene in especial strains causing viseral form was similar to previous reports of visceral leishmaniasis. It seems that this gene may play an important role in the visceral ability of specific strains of Leishmania tropica.
immunology & Biochemistry
Ezzat Nourizadeh
Volume 28, Issue 2 , May and June 2021, , Pages 302-310
Abstract
Introduction: Leishmania parasitic infections are the important causes of health problems in many parts of the world, especially in developing countries. Monoclonal antibodies have been used as valuable tools for the detection, treatment and characterization of the antigenic markers of parasites. This ...
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Introduction: Leishmania parasitic infections are the important causes of health problems in many parts of the world, especially in developing countries. Monoclonal antibodies have been used as valuable tools for the detection, treatment and characterization of the antigenic markers of parasites. This study through applicable techniques aims to produce mAbs against Iranian type of Leishmania( L). infantum. Materials and Methods: Standard strains were cultured and their antigens were used. BALB/c mice were injected with freeze-thawed promastigote twice together with Freund adjuvant. Three days before cell-fusion, the antigen through vein was injected into the mice. Then the mice were killed. After that their spleen lymphocytes were mingled with myeloma SP2/0. In the next step, the isolation of monoclones was performed by limiting dilution method. Results: 16 mAb against promastigote form of L.infantum parasite were obtained ,3 of which showed optical density (OD) more than 1nm, designated as 5D2 FV, 4G5 FV and 5D6 FIV. Then, anti-promastigotes L.infantum mAbs were obtained from these hybrids. These antibodies are effective in the logarithmic phase of the parasite. Conclusion: It seams these antibodies can demonstrate reaction against Iranian strain of promastigotes L. infantum and can be employed in the diagnosis of kalazar disease